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ACE C 18-AR
Benefits:
- Provides alternate selectivity to standard C18 columns recommended for compounds with aromatic functionality
- Ultra inert, ultra high purity silica, for excellent peak shape and reproducibility
- Compatible with highly aqueous mobile phases to enable the retention and separation of polar compounds
- Exceptional bonded phase stability for elevated temperature applications
- Ultra low bleed phase ensures UV and LC/MS compatibility
- Available in high throughput column dimensions
ACE C18-AR - a unique C18 bonded HPLC column offering
"ACE C18-AR is a unique C18 bonded HPLC column from Advanced Chromatography Technologies offering alternate selectivity to all existing C18 bonded phases. The phase combines the enhanced aromatic selectivity of a phenyl phase with the hydrophobic characteristics of a C18 phase, enabling the benefits of both interactions to be fully exploited. The phase exhibits ultra low bleed to ensure LC/MS compatibility and demonstrates excellent temperature and pH stability. Additionally the ACE C18-AR is suitable for use in highly aqueous (up to 100%) mobile phases to enable the retention and separation of polar compounds.
The material is available in 3um, 5um and 10um particle sizes and a full range of column dimensions from high throughput through to preparative scale.
Why do I need another new C18 phase?
The use of an ultra pure, ultra inert silica has many recognised benefits, including improved reproducibility, life time and chromatographic performance (particularly with basic molecules). However, since the ultra inert silica surface effectively no longer contributes to the separation, C18 columns manufactured with high purity silicas show near identical selectivity. It is therefore highly likely that a problem separation on one leading brand will not be significantly improved by changing to to an alternate manufacturer’s equivalent product
For many years, experienced chromatographers have been seeking phases with the proven performance and reproducibility benefits shown by such leading C18 column brands, but which additionally provide the alternate selectivity required for their challenging applications
When should I use ACE C18-AR?
Due to their similar hydrophobic characteristics, ACE C18 - AR columns may be used for application in which “standard” C18 columns would normally be considered. However, due to its integral phenyl functionality, ACE C18-AR is additionally recommended for separations that involve compounds containing aromatic functionality
As indicated below C18-AR provides an alternate selectivity to C18 columns, but remains a valid selection for methods in which C18 bonded columns are specified. In many instances, the same evaluation condition that prove unsuitable for the C18 compounds prove suitable for the C18-AR colunm
Example 1 Water soluble Vitamins
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