Features

• High performance - excellent peak shape for higher sensitivity
• Choice of 13 low bleed phases for complete optimization
• Ultra-inert silica enables MS compatible buffers to be used
• 20, 30, 35 and 50 mm column lengths
• 1.0, 2.1, 3.0, 4.0 and 4.6 mm i.d.s

Nowhere is the need for a truly ultra-inert base deactivated HPLC column more important than in high-throughput analysis or LC-MS. Even subtle differences in silanol activity between columns can markedly affect the chromatography in the very short, fast columns typically used. In addition, any peak tailing due to silanol activity can have a profound effect on detection limits in high sensitivity assays.

ACE ultra-inert base deactivated HPLC columns virtually eliminate the negative effects of silanols in HPLC separations. This unequalled performance is now available in 20, 30, 35 and 50mm length columns, with diameters from 1.0 to 4.6mm. The ACE columns are suitable for high throughput and LC-MS applications, and are the ideal choice for high volume screening assays used for drug analysis and combinatorial libraries where robust, reproducible columns are essential.

Increased Column Robustness

ACE LC-MS and rapid analysis columns are manufactured by a unique process which results in increased column bed stability, thus increasing column lifetime.  

LC-MS Buffer Compatibility

ACE LC-MS columns have an extremely low requirement for buffer salts or modifiers in order to achieve good peak shape. ACE columns have been demonstrated to be extremely robust at high and low pH. This broad pH operating range enables thorough optimization of pH and simplifies method development to a single column rather than selecting 3 different columns with low, intermediate and high pH stability.

For maximum LC-MS compatibility under acidic conditions organic acids such as formic, acetic and TFA are recommended. Under basic conditions ammonium bicarbonate, ammonium acetate and ammonium hydroxide buffers are recommended.

The Figure below demonstrates the resolution obtained with 2 basic compounds at different mobile phase pHs on an ACE ultra-inert HPLC column.