Benefits Rapid Method Development Kits

• Five 50 x 4.6 mm columns
• Wide range of phases
• Rapid economic column screening

The use of Rapid Method Development Kits allows the chromatographer to rapidly evaluate a series of bonded phases for routine analysis. Once the best partial or total resolution of the sample is obtained, when necessary a longer column packed with the same phase can be used to obtain optimum separation (Figure 1).

We offer >95% of the world’s leading brands of HPLC phases. Hence our Rapid Method Development Kits can contain an extensive range of columns packed with the chromatographer’s selection of phases (silica, chemistry and particle size). Please contact us for guidance on column selection or information on our applications support or column screening services.

Many leading manufacturers also offer competitively priced Method Development Kits including ACE, Kromasil chiral , NUCLEOSIL chiral  and YMC .

Benefits Selectivity Kits

• Three 150 x 4.6 / 250 x 4.6 columns
• Wide range of phases
• Optimum resolution
• Detect minor impurities

Chromatographers may wish to optimise the bonded phase on longer columns. Additionally, the use of all three columns within  our Selectivity Kits enables the chromatographer to further check for impurities that may remain undetected when only one column is used.  Figure 2 demonstrates the use of a Selectivity Kit containing three Vydac C18-bonded reversed-phase columns of differing selectivity aiding the detection of minor impurities in an antihistamine sample.

Selectivity Kits are available in a wide range of phases customised to the user’s needs.

Benefits Validation Kits

.• Three columns
.• One phase, three batches
.• Rapid method validation
.• Confirms assay reproducibility, robustness

Method Validation Kits contain three columns of the same phase and dimensions, but which are packed with different batches of material. The kits are available in all column dimensions and phases. They can be used to assess the batch to batch variability of a chosen bonded phase.
By validating a number of batches of material, the chromatographer can obtain greater confidence in the reproducibility and long-term robustness of the method.