Polar Bonded Phase

Introduction

Polar bonded silicas offer an alternative selectivity to alkyl bonded materials. In general they have a lower hydrophobicity but higher polarity. Cyano-, amino- and diol-bonded phases can be used in both normal- and reversed-phase modes.
In normal-phase they equilibrate more rapidly with the eluent than silica itself and are not deactivated by traces of water. 

Availability

Cyano-, amino- and diol-bonded phases are available from a number of manufacturers. Further polar bonded phases are available from ES Industries and Macherey-Nagel.
The cyano phase is commonly used in reversed-phase. Wide pore silicas are now available for the analysis of hydrophobic proteins. Due to its rapid equilibration it is very suitable for normal-phase gradient elution.
In addition to normal-phase separations, amino bonded material can be used in reversed-phase mode for the separation of polar compounds such as carbohydrates or as a weak anion-exchanger.
The diol phase can be used in both reversed- and normal-phase modes. Differing pore size materials are used in size-exclusion separations. 

Cyano Bonded Phases

Phase

Manufacturer

Particle Size
 (um)

Pore Size
(A)

Surface Area
(m2/g)

ACE CN

ACT

 

 

 

BetaBasic CN

Thermo Scientific

 

 

300, 100

  Capcell Pak UG CN

Shiseido

 

 

 

 Chromegabond BAS-CN

ES Industries

 

 

 

Chromegabond CN-BD

ES Industries

 

 

 

Chromegabond CN

ES Industries

 

 

 

Chromegabond CN/HS

ES Industries

 

 

 

Cosmosil CN-MS

Nacalai Tesque

 

 

 

Develosil CN UG

 Nomura

 

 

 

Exsil CN      

Grace Davison

 

 

 

Genesis CN

 

 

 

 

Hypersil CPS

Thermo Scientific

 

 

 

Hypersil CPS2

 

 

 

 

Hypersil BDS

 

 

 

 

CPS Hypersil GOLD CN

 

 

 

 

 Inertsil CN-3

GL Sciences

 

 

 

Kromasil CN

Eka Chemicals

 

 

 

LiChrosorb CN

  Merck  

 

 

 

LiChrospher CN

 

 

 

 

NUCLEODUR CN and CN-RP

Macherey-Nagel

 

 

 

NUCLEOSIL CN

 

 

 

 

Ultrasphere CN

Beckman

 

 

 

Waters µBondapak CN

Waters

 

 

 

Waters Nova-Pak CN

 

 

 

 

   HP Waters Spherisorb CN  

 

 

 

 

YMC CN

YMC

 

 

 

Zorbax CN

Agilent Technologies

 

 

 

Zorbax SB CN

 

 

 

 

 

 

 

 

 

Particle Size

Pore Size

Surface Area

(µm)

(Ã…)

(m2/g)

3, 5, 10

100, 300

300, 100

3, 5

150

200

5

120

300

3, 5, 10

120

180

3, 5, 10

100

475

3, 5, 10

60

475

3, 5, 10

60

550

5

120

300

5

130

300

3, 5

100

200

4

120

300

3, 5, 10

120

170

3, 5, 10

120

170

3, 5

130

170

1.9, 3, 5

175

220

5

100

450

5, 10, 16

60

540

5, 10

100

300

5

100

350

3, 5

110

340

5, 10

100

350

7

120

200

5

80

-

10

125

330

4

60

120

3, 5, 10

80

200

3, 5

120

300

3, 5, 7

70

330

1.8, 3.5, 5, 7

80

180

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