Chromatographic Separation Processes and their Application

This is the gateway to technical explanation of a wide range of chromatographic / biochromatographic processes

Chromatography:
Chromatography is used to analyze, identify, purify, fractionate  and quantify  compounds. A compound is a mixture of molecules of various molecular weight, functionalities and composition
Arrow1

Atoms : Ions : Small molecules     :          colloids                  

                                Cytochrom A:Endotoxin:Ferritin         Virus

10-10                               10-9                               10-8                              10-7m
1 Angstrom                      1 nm                              10 nm                            100 nm

100      101        102        103        104        105        106        107        108        109 g/mol       

Chromatography for analysis of compounds

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GC (click here)

 

 

 

 

 

 

 

 

 

SFC (Click here)

 

 

 

 

 

 

 

 LC (Elution LC)(Click here)

 

 Fractionation of molecules

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Chromatographic Modes

Elution Chromatography

Displacement Chromatography

Frontal Chromatography

Supercritical Fluid Chromatography  (SFC)

Simmulated Moving Bed Chromatography (SMB)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Application Methods

Buffer selection

 

 

 

 

Hydrophilic  Interaction Liquid Chromatography (HILIC)

 

 

 

 

 

 

 

 

 

 

 

 

 
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