Supercritical CO2 Liquid Chromatography (SFC)
Modern Supercritical CO2 Liquid Chromatography (SFC) is ideally suited for the separation, isolation and purification of a wide range of chiral and achiral small molecules, natural products and small peptides. Such molecules are important for the pharmaceutical, agrochemical, food, natural products (hemp), chemical and environmental industries.
SFC can be considered as another form of liquid and gas chromatography using carbon dioxide as a mobile phase.
Advantages of modern Supercritical CO2 Liquid Chromatography
- cost-efficient
- fast
- automated
- high productivity
- waste minimizing
- one machine for multiple applications
- sustainable, minimal energy use
- CO2 recycling and recovery
Our SFC range of instruments
- PIC-Lab Analytical SFC
- PIC-Lab Hybrid SFC
- PIC-Lab PREP 100 SFC
- PIC-Lab SUPER SFC
- PIC-Lab MAGNUM SFC
PIC-Lab Analytical SFC
PIC-Lab Analytical SFC system is a powerful tool for automated method development and routine analysis/QC/profiling of crude reaction mixtures, purification fractions, natural products extracts and final products. The system is offered with 10 or 20 columns running in parallel for faster analysis.
The PIC-Lab Analytical SFC models are versatile and fully automated analytical systems for routine analysis, profiling, method development and quality control of chiral and achiral small molecules, peptides, hemp and other natural products.
The PIC-Lab Analytical SFC 10 is designed for 10 columns as standard. With the PIC-Lab Analytical SFC 10 NT, 2 columns can be run in parallel, allowing for a set of 20 analytical columns.
Main features
- Automatic change of columns, 10-20 analytical columns, 6 preparative columns and up to 6 solvents.
- Instrument operation is controlled by a Program Logic Controller (PLC), providing high reliability and reproducibility.
- Easy-to-use software on a desktop station allows the user to program sequences, visualize data, and process. The software facilitates the sorting of methods for faster screening and automatic classification of results to speed method selection.
- Wide range of detectors: UV, ELSD, CAD, and single-quad mass spectrometers
- Fully automated acquisition by time or threshold for each detector
Please find details below!
PIC-Lab HYBRID SFC
The PIC-Lab Hybrid SFC is a “one device for multiple applications” system
The PIC-Lab Hybrid SFC models are designed for users who want to perform method development, preparative separations, and fraction analyses on a single system.
The compact PIC-Lab Hybrid SFC find application in the chiral and achiral separation of small molecules, peptides, hemp and other natural products.
The PIC-Lab Hybrid SFC 20, 10-100, and 10-150 are designed for micro-scale separations, milligram to gram scale, with flow rates from 20 to 150 mL/min, and are ideal for columns with inner diameters of 10 mm, 20 mm, and 30 mm.
The PIC-Lab Hybrid SFC series is also available as an open bed system.
Key features
- Individual channels for each analytical and preparative UEFC ensure reproducibility and robustness
- Automatic change of columns (9 analytical columns, 6 preparative columns) and up to 6 solvents
- Instrument operation is controlled by a Program Logic Controller (PLC), providing high reliability and reproducibility.
- Proprietary software, on a desktop station, allows the user to program sequences, visualize data and process it.
- Intelligent software facilitates the sorting of methods for rapid screening and ranking of results to assist the user in rapid method selection.
- Stacked and non-stacked injections are possible in manual, automatic and extraction modes
- CO2 recycling reaches 90-95% efficiency
- Wide range of detectors: UV, ELSD and single-quad mass spectrometers
- Fully automated detection by time or threshold
PIC-Lab PREP 100 SFC
PIC-Lab PREP 100 SFC-MS HT is a new generation of instruments, the “open bed collection system” It enables routine mass and UV triggered purification of small molecules, peptides and natural products important in drug discovery.
The instrument is designed for automated mg to multi-gram separations of chiral and achiral single molecules or libraries.
The configuration of the PIC-Lab PREP 100 SFC-MS HT is designed to allow automated analytical method development and automated analysis of collection fractions.
In the configuration where the open bed module is removed, the PIC-Lab PREP 100 SFC processes separations one substance at a time.
Key features
- – Proprietary GLS technology ensures accurate and clean collection, allowing fraction tubes to fill up to 95% of capacity and supporting CO2 recycling at a maximum flow rate of 100 ml/min.
- – Separate injection and collection: For optimal speed and reliability, the injection system is separate from the collection module. Both the injection and collection modules have a flexible layout that accepts 96-well plate footprints or racks to suit your needs.
- – Stacked and non-stacked injections: Non-stacked injections are ideal for library purification. Stacked injections can be performed with the injection robot to enable rapid singleton separation. Alternatively, stacked injections are possible with the collection robot when automated separation of half a dozen or more chiral/achiral compounds is required, each requiring multiple injections.
- – All system operations are controlled by a programmable logic controller (PLC), which ensures high reliability and robustness. User interface to the instrument for sequence programming, fraction tracking and data visualisation is via the user-friendly proprietary software PIC Solution.
Details can be found below!
PIC-Lab SUPER SFC
The PIC-Lab SUPER SFC series is suitable for large scale purification of enantiomers and complex mixtures of small molecules, peptides, hemp extracts and other natural products. For larger scale purification, efficient and sustainable supercritical CO2 liquid chromatography (SFC) drastically reduces operating costs as it is faster, CO2 recyclable, uses less energy and much less solvent compared to HPLC.
The fully automated systems are equipped with standard detectors and a co-solvent pump to enable isocratic or gradient methods. Configurations with a single quad mass spectrometer are also available on request.
Key features
- – CO2 diaphragm pump is highly accurate, durable, reliable and highly efficient
- – Stacked injection with seamless injection module for fast and reliable cycles
- – Back-pressure regulator based on a robust and intelligent PIC solution
- – Detection with standard UV, ELSD, CA detectors or single quad mass spectrometers
- – Operation of the instrument is controlled by a Program Logic Controller (PLC), which provides continuous, reliable, robust and unattended operation. PIC Lab’s own user-friendly software is used for method programming and data visualisation.
- – Deliverables:
- PIC-Lab SUPER SFC 200, Maximum flow rate: 200 ml/min, Column i.d. 30 mm, 50 mm, Estimated throughput/day 15-500g.
- PIC-Lab SUPER SFC 400, Maximum flow rate: 400 ml/min, column i.d. 50 mm, 76 mm, Estimated throughput/day 45-1000g
- PIC-Lab SUPER SFC 600, Maximum flow rate: 600 ml/min, column i.d. 50 mm, 76 mm, Estimated throughput/day 90-1500g
- PIC-Lab SUPER SFC 800, Maximum flow rate: 800 ml/min, Column i.d. 50 mm, 76 mm, Estimated throughput/day 135 -2000g
Please find details below!
PIC-Lab MAGNUM SFC
The PIC-Lab MAGNUM SFC series is designed for industrial-scale purification of enantiomers and complex mixtures of small molecules, peptides, hemp and other natural products.
For larger scale purification, efficient and sustainable supercritical CO2 liquid chromatography (SFC) drastically reduces operating costs as it is faster, recycles CO2, uses less energy and much less solvent compared to HPLC.
Key features
- – Fully automated systems
- – CO2 diaphragm pump is highly accurate, durable, reliable and highly efficient
- – Stacked injection with seamless injection module for fast and reliable cycles
- – Back-pressure regulator based on a robust and intelligent PIC solution
- – Detection with standard UV detectors
- – Unit operation is controlled by a Program Logic Controller (PLC) ensuring continuous operations, reliable cycles and robustness.
- – Deliverables:
- PIC-Lab MAGNUM SFC 10, column size (i.d.) 10 cm, Estimated throughput/day 1-5 kg.
- PIC-Lab MAGNUM SFC 20, column size (i.d.) 20 cm, estimated throughput/day 4-20 kg
- PIC-Lab MAGNUM SFC 30, column size (i.d.) 30 cm, estimated throughput/day 8-40 kg
Please find details below!
PIC Solution Software
The PICLab software is available on a separate PC and allows the user intelligent sample programming, automated sample runs and fast data visualisation.
PICLab software is developed by scientists and interfaces with the Program Logic Controller (PLC), which manages all instrument module operations to ensure high reproducibility and robustness.
Select the system you want and request a quote
