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Knowledge in Chromatography

Knowledge in Chromatography is a key factor of success!

Dear Visitor,  Knowledge in Chromatography is very important

Indeed, if you do not understand the underlining processes you cant create productivity and reproducibility!. You become dependent on large equipment suppliers that supply expansive and robotised equipment.  the danger is real that you will be replaced one day by machines. Do not forget, Knowledge in Chromatography is a key factor of success. It is difficult to know at what level your knowledge is.

Our priority is to assist our clients to become specialists and independent by supplying product linked knowledge. The knowledge section supplies product linked and general knowledge. The latter has aimed at a broad range of clients and potential clients.

Chromatography has expanded into a crucial scientific research tool, modern technology and production technique. Moreover, throughout the previous 60 years chromatography has been continuously advanced.  It is the preferred separation and purification technology to effectively and efficiently resolve a significant number of different molecules and particles by a wide variety of procedures.

Knowledge in Chromatography is divided into several sections

In the knowledge part, we look at Chromatography from different perspectives to get some structure into a large field of knowledge.  Clicking the button below will bring you to the relevant section 

 Description of Techniques by Chromatography bed shapes

brings you to column-based HPLC processes.

Column based HPLC Chromatography Processes

Most Chromatographic purification/separations processes are done in columns. Furthermore, everybody has a different method of learning and discovering. There are Video presentations according to your depth of knowledge. They are targeted to Senior High School Scholars to Technicians to PhDs and finally for Post Docs

Monolithic Column Chromatography

Planar Chromatography

Expanded (Fluidized) Bed Adsorption

Bio Adsorption Chromatography

Flash Chromatography and MPLC


Description of Techniques by physical state om mobile phase

Theorie Überkritische Flüssigkeit Chromatographie

 Theory Supercritical Fluid Chromatography

Gas Chromatography

Description of Techniques by the separation mechanism

Normal Phase Liquid Chromatography

Reverse Phase Liquid Chromatography

HILIC and ANPC Chromatography

ERLIC Chromatography

Hydrophobic Interaction Chromatography

Affinity Liquid Chromatography

Ion Exchange Chromatography

Ion Chromatography

Multi (Mixed) Mode Chromatography

Size Exclusion Chromatography


Metabolomic Analysis

Protein Variant Analysis

Haemoglobin Variant Analysis

Chiral Separation Chromatography

Process Scale Chromatograph

Library of start-up Methods

Chromatography Start-up-Methods Library 

This section is of wonderful assistance for Method Developers.  In this section, you find a huge variety of start-up methods or you might compare the methods from different suppliers. It empowers you to determine costs differences. Please send us your methods by Contact Page for insertion into the Method section

 Skill Improvers  

This area is for everybody that is practising Chromatography. Just check it, you might discover some technique to improve your skills and effectiveness or you might find some information for everyday use. We also appreciate receiving your skill improvers by Contact Page

Dear Novice

Many arrive here driven by an interest in Chromatography.  The video below is a good introduction to what chromatography is.

With Knowledge, you can differentiate yourself from others.

Have you ever before considered the pressures behind a commercial success? Pressure forces are people like you and me, unique People! You might be a Worker in a firm or maybe in academia. Alternatively, you might be a proprietor of a company or a pupil in the High School. Have you ever asked, “What makes my or a job  in chromatography secure as well as satisfying?”

Everybody is hired with ideas that he/she will create value for his company or institution. The owners have already invested in raw material, wages/salaries and overheads! So, what is your contribution?

If you are being hindered to create value or being productive by somebody and you are not allowed to improve your skills and technological expertise – then you should change your employer!

What is your background?

In academia, you are either a researcher or lecturer. You develop value by discovering or designing brand-new knowledge, – expertise or applications that can be IPR protected. The dimension of the created worth depends on whether a customer can be found including how much he is ready to pay for the transfer of your know-how.

You may be a pupil that just aims to pass exams. There may be no interest in developing anything of worth. However, in a performance-oriented world value developers have much higher career chances and job security.

Assuming a business proprietor does employ people that don’t produce substantial value, then his company or your job will not last very long.

Not everybody has the competency to create value

Lots of supervisors prefer to reduce costs over the creation of new and better products because it is simpler. You know it is popular to minimize staff or to pressurize distributors for discount rates.  Unfortunately, cost-saving is an extremely limited game.  With saving strategies, the results are typically not spectacular. In fact, in the pharmaceutical industry cost have increased from USD 450 Million/ for every new NCE/NBE in 1995 to USD 4.8 Billion by 2015.

Developing worth requires knowledge, understanding, strategy, perseverance and technique.  Creating value for the market will attract more clients and revenue. Extending patent life by say two years has a high chance to create additional earnings in terms of billions.

Chromatography Knowledge is a key factor in success?

Unfortunately, many chromatographers as well as medicinal chemists are not enjoying long term job security. On the other hand, many don’t care to be used to commoditize their workplace. The smart ones make sure they specialize in their work. Commodities are candidates to be conveniently exchanged by robotic devices and unskilled labour.

In this Knowledge Section, we present tools that empower you to become more effective and efficient in your job. It is a fact, the industry can enjoy the fruits of previous labour. The industry today is growing well because in the past numerous tiny and extremely versatile and specialized businesses teamed together with individuals working in the pharmaceutical and biotech companies.  Together they created several special tools or one-of-a-kind services and products. Today we mainly must finetune and expand the technologies we have. However, we should also develop new applications,

Unfortunately, many large life science companies and institutions have been acquired by hedge funds. They are not known to develop the industry further. They are known to monopolize and commoditize their acquisitions to increase their profitability. 

There are approx. 7000 uncurable diseases. Life Sciences grow only with competitive and effective new drugs and devices.

Hedge funds outsource everything that carries a high risk particularly R & D.  They are great in outsourcing innovation to universities. There is a belief amongst finance people that expansive specialists can be easily replaced by robots and artificial intelligence.  Unfortunately, in medicinal chemistry, we have only very few laws of nature or algorithms that can be used to create complicated bioactive molecular constructs and devices. Furthermore, Universities generate “proof of concepts” and publications but very few tangible and valuable hits and leads.

What is missing in a hedge fund driven world are highly motivated and creative business owners.  Most business owners have pioneered something. They spot business opportunities and convert them into problem solutions with passion and tenacity.  In the process, they become owners of their creations.

Large pharmaceutical companies have become pools of people with widely diverting contradicting interests. Instead of fixing productivity, the industry has created a rational reason (EROOM – Law) to explain declining productivity.

Product growth in most large companies is stagnating in the western hemisphere

Not surprising that the sector is currently on a growth plateau in the product life cycle. Plateau formations are not sustainable, they either return to additional growth with a new point of view, new technologies or products or they decline.  Following that they are being acquired by a stronger player. Some simply disappear at some point due to inactive experts or due to the emergence of disruptive technologies.

Often new managers come into some large pharmaceutical companies. Their task is to create new blockbuster drugs. Most know that this is possible only via ingenious as well as motivated hard-working technical individuals. However, if in previous years whole departments have been closed and the specialist replaced with machines and low-cost labour then it takes many years to bring the company back into a growth phase.

Tangible technical skills and Chromatography Knowledge is a key factor of success,

Studying profiles of outstanding achievers in the life sciences reveals Impressive technical people are very ambitious, rather introspective individuals. They are no team players that require a large degree of freedom. Finding such a leader has become very difficult. Furthermore, in the Manufacturing and Work 4.0 world, such exceptional people are not considered cool. Modern Managers don’t appreciate autocrats or disruptors – they want conformists that can be managed easily 

It is popular amongst Managers to assert leadership skills.  Many convince investors to modernize the company culture.  The reality is that everybody is unique and ambiguous. The larger an enterprise the more diverse the range of ambition within an enterprise.  In hierarchical systems, the manager controls the top down. In democratic systems, everybody is supposed to manage himself and to interact with others and accept tasks from others. Large companies have gradually removed their owners that skilfully directed many Managers and workers. Today even the board of directors are representing and not real owners. 

To get the company moving in the right direction within a democratic system requires leaders the people trust.  Everybody makes some mistakes and people trust only emphatic leaders that don’t hide their mistakes. Unfortunately, in the mid-eighties, investors have created “short-termism” and corrupted the economic system. Since then large enterprises have had no visible leaders anymore. The images and profiles published through the press are created construct that applies to a small group of players. 

Fundamentals of progress

There is a wide range of strategies to create wealth.  However, in life science and health-based companies have a mission to eliminate crippling diseases, to improve health and quality of life. Thus, all resources should be bundled to achieve this mission through diagnostics, therapeutics, medical devices and procedures.  In a competitive world, those products and processes must be outstanding and unique. And that, in turn, requires first and foremost creative and motivated people with a huge drive to create tangible solutions.

There are many knowledgeable and skilled individuals in society capable of producing tangible results. Unfortunately, the political, educational and financial system has created constructs to control the creators as puppets on the string. Venture Capitalists make sure that the resulting problem solutions is controlled and owned by the VC, Investors, Advisors but not by the Creators.

A few very rich entrepreneurs have created alternative strategies by motivating and financing their sons and daughters to create new diagnostics, therapeutics, medical devices and procedures. Those who started a drug discovery failed because they did not get the relevant governmental support.  Thus, those who started quietly and with a less complex system (shorter time to the market) succeeded.

Partnering with creative SMEs.

Everywhere around the globe some individuals create some new concepts, methods or products. To transform the status, large companies should go back to cooperation with technically competent pioneers and very inspired business owners that have funds to develop new technologies.

Such cooperation is possible if the managers stop manipulating the system.  SMEs need trustworthy partners with global distribution power and a strong flow of revenues.  If both parties reach consent on long-term strategies and agree not to exploit each other then the basis for the win-win situation is created.

If such a constellation can not be created disruptive players will commoditize the industry further and offer low-cost psychosomatic software packages that promise well being and long life.  Such competition will not bring tangible problem solutions but confusion and disarray.

What is your proposed strategy to get the life science and health sector back on track?  Please forward your ideas by Contact Page