Description
Structure of Product Description: Column Name Particle diameter(um) Functional Group Column length(mm)x ID(mm)/Art No.
Description
Properties
Vydac TP phase specifications
Phase: Vydac 219 TP
Phase description: Vydac 219 TP Diphenyl
Particle Size (um): 5, 10
Properties: Lowest capacity first generation diphenyl phase
Application Benefits: Polypeptides with aromatic side chains, large hydrophobic proteins, membrane-spanning peptides, lipid peptides, fusion proteins from inclusion bodies
USP Code: L11
Vydac 219TP is suitable for polypeptides with aromatic side chains, large hydrophobic proteins, membrane-spanning peptides, lipid peptides and fusion proteins from inclusion bodies.
Vydac MS phase specifications
Phase: Vydac 219 MS Dipheny
Particle Size (um): 5
Properties: Lowest capacity, aromatic functional groups
Application Benefits: Highly selective for proteins with aromatic side chains
USP Code: L11
Vydac 219MS has the lowest capacity and is highly selective for proteins with aromatic side chains.
Difference between TP and MS
Vydac TP
Vydac TP was the first range of wide pore media phases developed in the Vydac range and became the industry standard for peptide and protein separations. The large pores of the 300A TP silica give polypeptide molecules complete access to the interior of the silica pores.
The general features of Vydac TP include:
Long column lifetime and negligible phase leaching
Reliable protein purifications, scalable from analytical to preparative scale
Referenced in a large number of patents and publications
Vydac MS
Vydac MS columns are a later development from Grace for the reversed-phase HPLC separation of biomolecules. A proprietary surface treatment and bonding process give Vydac MS columns unique selectivity. A variety of reversed-phases makes this product line suitable for the analysis of small peptides to large intact, undenatured proteins.
The general features of Vydac MS include:
300A pore size spherical silica
Five reversed-phase chemistries
Excellent peak shape with little or no TFA
High protein recoveries make scale-up easy
The improved selectivity for peptides on the Vydac MS columns results in better primary structure definition and easier identification of degradation products and other protein characteristics.
