Description

Explanation

for Vydac 238TP & 238MS Column

Product Description in the window above has the following structure: Column Name Particle diameter(um) Functional Group Column length(mm)x ID(mm)/Art No.

Properties of Vydac 238TP & 238MS Column

Vydac TP phase specifications
Phase: Vydac 238TP
Phase description :Vydac 238 TP C18)
Particle Size (um): 3, 5, 10
Properties: First generation monomeric C18 phase with unique selectivity
Application Benefits: Use for same applications as 218TP, but offers different C18 selectivity
USP Code: L1

Vydac MS phase specifications
Phase: Vydac 238 MS C18
Particle Size (um): 5,
Properties: Polymeric bonding, highest hydrophobic interaction and unique geometric selectivity
Application Benefits: Use for same applications as 218MS, but offers different C18 selectivity
USP Code: L1
Vydac 218MS and 238MS are used for simple enzymatic digests (< 12 proteins) or biomolecules (0-5kDa).

Difference between TP and MS

Vydac TP

Vydac TP was the first range of wide pore media phases developed in the Vydac range and became the industry standard for peptide and protein separations. The large pores of the 300A TP silica give polypeptide molecules complete access to the interior of the silica pores.

The general features of Vydac TP include:
Long column lifetime and negligible phase leaching
Reliable protein purifications, scalable from analytical to preparative scale
Referenced in a large number of patents and publications

Vydac MS

Vydac MS columns are a later development from Grace for the reversed-phase HPLC separation of biomolecules. A proprietary surface treatment and bonding process give Vydac MS columns unique selectivity. A variety of reversed-phases makes this product line suitable for the analysis of small peptides to large intact, undenatured proteins.

The general features of Vydac MS include:
300A pore size spherical silica
Five reversed-phase chemistries
Excellent peak shape with little or no TFA
High protein recoveries make scale-up easy

The improved selectivity for peptides on the Vydac MS columns results in better primary structure definition and easier identification of degradation products and other protein characteristics.