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Ion Exchange Chromatography

Ion Exchange Chromatography is an important separation and purificatchion technology partticularly in analytical, prep and process scale Biochromatography. Ion Excchange Chromatographys is an umbrella term that can be divided in three modes  and three types of phases  1. Anion- 2. Cation and 3 Mixed Bed Ion Exchange phases all using conductivity detection.

Ion Exchange Chromatography Video Clips

       Ion Exchange Chromatography (English) Fundamental     

               Ion Exchange Chromatography (English) Basics            

I Ion Exchange Chromatography (English) Technician Level      

      Ion Exchange Chromatography  (English) Technician Level  

Ion Exchange Chromatography (English) Post Graduate Course



Ion-exchange phases separate solutes on the basis of ionic charge. Retention in ion-exchange chromatography is determined by the pH of the eluent, the nature and ionic strength of the buffer and temperature. Column efficiencies are lower than in reversed-phase HPLC. Eluents are normally aqueous but can contain some organic component.
Base Material

Both silica-based and polymer-based ion-exchangers are available. For the former, ionic species are attached to the silica surface, whereas for the latter the ion-exchange groups are distributed throughout the matrix. Silica based materials maintain a mechanical strength and higher efficiency advantage whereas the polymer based materials have greater pH stability.

Ion-exchange is used for the analysis of small ions but its key application area is in the separation of biomolecules such as proteins and nucleic acids. Weak ion-exchangers are used for the analysis of inorganic ions, a technique more specifically termed ion-chromatography.
Ion-Exchange Capacity

The exchange capacity of an ion-exchanger is an important measure of its retentivity (typically measured in milliequivalents per gram material). For any one column the packing density of the phase must also be taken into account. Wide pore materials will typically have lower ion-exchange


Mixed Bed   http://www.polylc.com/Downloads/Mixed-bed_IEX_bulletin_intact_proteins.pdf

Catiion Exchange  http://www.polylc.com/downloads/ISPPP_1996_CEX_poster.pdf  

Anion exchange:   http://www.polylc.com/downloads/Nucleicacidsbulletinrev36-3-03.pdf