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SieLC mixed mode specialist

SIELC mixed mode specialist


SIELC Technologies is a mixed mode specialist and pioneer in creating highly innovative HPLC materials and columns. Two highly creative chemists formed the company Vlad Orlowski and Uri Seletionec in Prospect Hights, Il, north of Chicago. In the meantime, Vlad moved on to form his own company Horizon

Nevertheless, SieLC grew further as highly innovative specialist. They expanded by addressing difficult separations with novel technologies and methods. 

We know SieLC and distribute their products since their Commencements.

Product Range  

FliPLC to Remove Impurities

Cool Methods

NewNovel Hardwarew and DeviceChrom novel Ion Exchange Column

Legacy Columns to Match USP Methods  

Obelisc to improve Productivity in HPLC  

Primesep Multi Mode HPLC Column      

Promix Biomolecule HPLC Columns       

Shark Column Utilizes Hydrogen Bonding    


Why should you buy SieLC Products

In a world of progress there is competition. To escape competition  specialist develop permanently new problem solutions. Furthermore, the problems become increasingly complex. Modern  Pharmaceutical molecules  cary in the same construct hydrophibic, hydrophilic, ionic and chiral domains, Similar with phytochemicals.

In some companies there is also a philosophical fight. Some purchasing manager argue that all Chromatography can be done with thre to fife commodity column.  The rational behind this philosophy is  masspurchsing at the lowest price. To have simple method and low cost staff doing the work.

Professional chromatographers on the other hand create unique Application methods. That in turn requires unique columns. With better column section and multifunctional columns they make a company and their products more uniques. Uniqueness can be IPR protected. This way whe helped a few companies to expand patentlife from 25 to 27 years. In pharmaceutical industry this will generate additional revenue in terms of billions.  At the same time such a move has also a strong influence on job security and promotion of the responsible chromatographer.  

Unfortunately, in recent years in a rush of acquisition frenzy a number of patet extensions were destroid by carless handling of IPR.

Smaller life science companies grow by creating highly differentiated specialties using more complex production tools. We are also interested to evolve special materials either as market control factor or as an IPR tools.

SieLC is permanently pioneering new applications and thus helping professional chromatographers to improvr their job security.

Polyethyleneimine (PEI) by mixed mode HPLC analysis

polyethyleneimine (PEI) has multiple industrial, medical, biological and research applications. It is a difficult compound to analyse by HPLC. The problem has many degrees of difficulty.
PEI is not a monodisperse compound. It is a mixture of different molecular lengths and branching structures.
It has multiple charges in acidic and neutral pH, which is most common in HPLC
PEI molecules have no UV chromophores. In other words, UV-Vis detector cannot measure them. Incorporating a Cu* complex empowers you to use a UV-Vis Detector. For more about this method click here

NewChrom is a new type of mixed-mode HPLC columns

SieLC attached long alkyl chain to the silica gel surface.  The ion-exchange group is at the terminal end of this chain. Consequently, the ion-exchange functional groups have high mobility inside the pores of the solid particles.  This ends the chelating (trapping) of multi-charged analytes on a surface of traditional ion-exchangers. The embedded ion-exchange groups in Newcrom behave as ions in a solution. However, they are mobile, but they cannot go beyond the length of the ligand chain. Read more.

Frequently Asked Questions

What are Mixed or Multi mode materials?    

You find more theoretical and practical knowledge in here  

   MSD for Silicat

Frequently Asked Questions about Column/Material

  1. g Why can’t I use reversed-phase HPLC for proteins?

2) Do I need to degas mobile phases?

3) How should I store columns?

4) What’s the correct way to prepare a mostly organic mobile phase?

5) Why aren’t my peptides retained on the PolySULFOETHYL A™ column?

6) Can I use TFA or HFBA in the mobile phase using PolyLC for Bio HPLC?

7) My peaks are badly skewed or split in HILIC. Why?

Frequently asked Company Admin Questions


How to order

 Formulating a complete  Article/Item Number

Ordering combinations that are not in the catalogue



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